摘要
以我国乙型脑炎病毒疫苗株SA14-14-2的基因组RNA为模板,设计1对包含prM基因完整编码区的引物,采用一步法RT-PCR技术扩增其prM基因,扩增出大小约558bp特异性带。将其克隆到pMD18-T载体中,用双脱氧末端终止法进行全序列测定,序列分析表明我国疫苗株SA14-14-2的prM基因核苷酸序列与GenBank收录的完全一致。prM基因的获得为进一步研究该基因的免疫原性以及乙型脑炎病毒基因工程疫苗奠定了基础。
Viral genomic RNA was separated from JEV attenuated live vaccine. The cDNA encoding precursor membrane(prM) protein was amplified by one step reverse transcription polymerase chain reaction(RT PCR) method using seperated viral genomic RNA as template and cloned into the pMD18 T.By comparing with JEV sequence in GenBank,the homology value was 100%.The obtainance of prM gene of JEV laid a basis for further study on immunogenicity and gene engineering vaccine of prM gene.
出处
《动物医学进展》
CSCD
2003年第1期87-89,共3页
Progress In Veterinary Medicine
基金
九五重中之重科技攻关项目(96-003-01-041)
