摘要
目的 :探讨小细胞肺癌与 [Tyr3 ]octreotide (TOC)的亲和力。方法 :用氯胺T方法标记的12 5I-TOC作为配基 ,检测小细胞肺癌细胞株NCl-H4 4 6与其结合的受体位点数及亲和常数。结果 :氯胺T法标记的12 5I-TOC经SephadexG - 10纯化后放化纯大于 95 % ;受体分析显示NCI-H4 4 6表达的生长抑素受体多 (Bmax =1 17× 10 5受体位点 /细胞 ) ,与12 5I-TOC亲和力强 (Kd =0 5 8nM)。结论 :TOC的标记物可用于小细胞肺癌肿瘤的受体显像及放射治疗。
Objective To study the affinity of small cell lung carcinoma to octreotide (TOC). Methods Taking 125 I-octreotide (labeled by chloramine-T method), as the ligand, small cell lung carcinoma NCI-H466 cell line was inspected for the receptor-binding points and affinity constant. Results The radio-chemical purity of 125 I-TOC purificed through saphedex G-10 was higher than 95%. Receptor analysis study showed that the expression of somatostatin receptors on NCI-H446 cells was numerous (Bmax=1.17×10 5/cell) with strong affiny to 125 I-TOC (Kd=0.56nM). Conclusion Labeled TOC could be used for small cell lung carcinoma receptor imaging and radio-pharmaceutical therapy.
出处
《放射免疫学杂志》
CAS
2002年第5期277-279,共3页
Journal of Radioimmanology
