摘要
目的:建立一种测定银杏叶提取物的高效液相色谱方法。方法:样品经简单处理后,用甲醇-水-冰乙酸(40:57.5:2.5)为流动相及C_(18)色谱柱,在254nm波长处检测,以芦丁为对照计算总黄酮。结果:该方法线性关系优良,加样平均回收率为99.41％。RSD为1.32％。结论:此方法快速、准确,亦可作为提取物制品的测定方法。
Objective: To develop p HPLC method of EGB. Method: After treating sample simlely, Mobile phase: methanol-water-glacial acetic acid (40: 57.5: 2.5) and C_(18) chro- matographic column, detection wavelength: 254nm. Calculate flavonoid and compare as rutin. Re- sult: this method linear is fine. the average rate of recovery is 99.41%, RSD: 1.32%. Conclusion: This method is quick and accurate, also as determination method of estract products.
出处
《黑龙江医药》
CAS
2002年第6期426-427,共2页
Heilongjiang Medicine journal
