摘要
目的:制备骨基质支架并与自体红骨髓联合移植修复兔桡骨节段性骨缺损,探讨该支架作为骨组织工程载体的可行性。方法:采用NaOH消蚀技术,制备出家兔骨细胞外基质支架,行扫描电镜观察及生物相容性实验,并与自体红骨髓联合移植于兔桡骨节段性骨缺损模型,通过X线、生物力学等检查,比较其联合红骨髓及单纯支架移植修复节段性骨缺损的疗效。结果:(1)经NaOH消蚀处理后,骨组织中的细胞成分被彻底清除,细胞外基质成分-胶原纤维仍维持三维立体网状结构;无明显排异反应且可降解吸收。(2)支架+自体红骨髓修复骨缺损,4周即出现成骨现象,8周可修复骨缺损;单纯支架移植,约在12周修复。生物力学测试结果,复合红骨髓组抗压强度大于对照组(P<0.05)。结论:此方法制备的骨基质材料与红骨髓联合,可有效地修复节段性骨缺损,是理想的骨组织工程天然载体。
Objective: To prepare a new natural grafting material for bone transplantation. Methods: The material was made by immersing the shaft of radius of rabbits in NaOH aqueous solution and then was observed by scanning electron microscope (SEM). The materials were implanted into the muscles of rabbits for the study of tissue reactions in vivo. Then the material was-combined with autologous red marrow (ARM) to repair 15mm segmental defects created in the radius of rabbits. The material alone was control-group. The defect-repairing capability for each of the treatment modalities was assessed radiographically and biomechanically. Results: The cellular elements of the bone treated with NaOH-maceration were removed effectively and consistently. The extracellular matrix that was consisted by collagen fibrils and minerals was preserved in their natural position. The 3D architecture of collagen fibrils can provide a microen-vironment which was suitable for cells function. The mineral can make the frame more strength in mechanics. Histological examination revealed that there was nearly no tissue reaction during the implantation and the grafted frames were absorbed gradually. The material and ARM exhibited a strongest defect-repairing power and apparent healing of the defect can be seen after 8 weeks. It was earlier than the control. Conclusions: The bony frame prepared by NaOH-maceration provides an excellent material for the reparation of segmental bony defects. It can be used as a carrier for the bone tissues engineering.
出处
《中国临床解剖学杂志》
CSCD
北大核心
2003年第2期163-166,共4页
Chinese Journal of Clinical Anatomy
基金
河北省自然科学基金资助项目(302492)
关键词
细胞外基质
骨缺损
骨髓
实验研究
extracellular matrix
bone defect
bone marrow
experimental study
