摘要
本文构建了一个包含 16 12 8个克隆的稻瘟菌基因组的TAC文库 ,74%的克隆含有外源插入片段 ,其插入片段大小平均为 5 9kb ,该库相当于稻瘟菌基因组的 18倍。本文并对文库的代表性进行了评价 ,采用 3个单拷贝的标记作为探针筛库 ,分别得到 16 ,17和 16个阳性克隆 ,这与估算的该库的覆盖率是一致的 ;从MH18S1为探针所筛选的阳性克隆中 ,随机挑取 8个并对其限制性酶切图谱进行分析 ,结果表明 :其组成的重叠群跨度为 15 3kb ,未发现嵌合和缺失现象。以上结果表明 ,此TAC文库适合用于稻瘟菌基因组的分析。
A TAC library of rice blast fungus, Magnaporthe grisea, was constructed in a vector pYLTAC7. The library contains 16 128 individual clones and approximately 74% of the clones harbor foreign DNA inserts with an average size of 59 kb. The content of the library corresponds to 18 equivalents of the genome. To confirm the representative of the library, three single-copy DNA markers were used as probes to hybridize with the library clones on the high-density membranes. Sixteen, 17 and 16 positive clones were obtained respectively from the library screening, and the numbers of positive clones matching corresponding probes are consistent with the estimated coverage of the library. Further analysis of the restriction enzyme maps of 8 positive clones screened with a single copy marker, MH18S1, revealed that the contigs covered a 153 kb region without chimerism or deletion. It is suggested that the TAC library is suitable for genome analysis of M. grisea.
出处
《植物病理学报》
CAS
CSCD
北大核心
2003年第1期57-62,共6页
Acta Phytopathologica Sinica
基金
theNational"863"Project (2 0 0 1AA2 2 30 81)andtheNational"973"Project(G2 0 0 0 0 1 62 0 1 )
