摘要
有机硫氰化合物二硫氰基甲烷 (TH 88,浸种灵 )可抑制水稻恶苗病菌 (Fusariummoniliforme)对多菌灵 (carbendazim)的抗性菌株和敏感菌株 ,EC50 在 0 3933～ 1 6 412 μg/ml之间 ,对菌丝和分生孢子的形态没有影响 ;该药剂 (浓度为 1μg/ml)对菌体的生物膜(透性 )有一定抑制作用 ;用药剂处理萌芽期、非萌芽期的分生孢子及其初形成的菌丝 ,结果表明 :药剂对菌丝和分生孢子的呼吸作用有影响 ,以分生孢子萌芽期最为敏感 ,用二硫氰基甲烷 2 μg/ml处理刚萌芽的分生孢子 5min ,呼吸作用比对照低 6 4 15 %;处理NADH细胞色素还原酶和NADH细胞色素氧化酶 ,前者没有影响 ,但对后者有 5 4 32 %的抑制。用二硫氰基甲烷 (1μg/ml)处理啤酒酵母 (Saccharomycescerevisiae)的线粒体 ,呼吸控制速率 (RCR)比对照低 2 7 6 7%;磷氧比 (P/O)比对照低 9 3%。
Organic thiocyanic chemicals, methylene dithiocyanate (TH-88, chemical formula: SCN-(CH 2) 2-SCN) can control rice 'Bakanane' disease caused by Fusarium moniliforme, both carbendazim -resistant and -sensitive strains. The EC 50 of TH-88 varies from 0.393 3 to 1.641 2 μg/ml. Biochemically, this chemical increases mycelium seepage with no effect on mycelium and conidium at the concentration of 1 μg/ml, but decreasing the respiration of mycelium and conidium of F. moniliforme, especially during the period of conidium germination with the decrease of 64.15% compared with CK 5 minutes after treatment at the concentration of 2 μg/ml. Methylene dithiocyanate doesn't inhibit the activity of NADH cytochrome C reductase, while it decreases 54.32% of NADH cytochrome terminal oxidase activity at the concentration of 2 μg/ml. Respiration control rate and the rate of P/O are 27.67% and 9.3% lower than CK, respectively, when the mitochondria of beer yeast (Saccharomyces cerevisiae) was treated at the concentration of 1 μg/ml.
出处
《植物病理学报》
CAS
CSCD
北大核心
2003年第1期48-51,共4页
Acta Phytopathologica Sinica
