摘要
目的 :观察死胎胸腺组织中HBVDNA表达的情况 ,探讨通过产妇传播到死胎胸腺组织中的乙型肝炎病毒(HBV)是否存在复制。方法 :采集 40例乙型肝炎产妇产下的死胎 ,常规尸检 ,取胸腺组织。采用原位分子杂交法 ,检测其中的HBVDNA ;回访产妇产前静脉血HBV的检测结果。结果 :死胎胸腺组织中HBVDNA的检出率为 2 7/ 40 ( 68% )例。产妇产前静脉血HBV呈高、低和无复制状态时 ,胸腺组织中HBVDNA的检出率分别为 14/ 16( 88% )例、11/ 2 0 ( 5 5 % )例和2 / 4( 5 0 % )例 ;高、低、无复制者之间相互比较 ,差异无显著性 (P >0 .0 5 )。HBVDNA颗粒分布在胸腺细胞浆内、细胞间和毛细血管内 ,少数分布在胸腺细胞核上。结论 :死胎胸腺组织中有HBV复制 ;
Objective: To observe the expression of HBVDNA and to determine the replication of HBV in the thymus tissue of the died fetus that delivery by pregnant women infected with hepatitis virus B.Methods:40 died fetus were made an autopsy for the thymus tissue and detected HBVDNA with in situ molecular hybridization technique.The replication of HBV was detected from the pregnant women before labor.Results:For the thymus tissue,27(27/40,68%)cases were detected out HBVDNA.When the replicated status of HBV in the maternal was high,low,and even none,expression of HBVDNA in the thymus tissue of the fetus was 2/4(50%),11/20(68%),and 14/16(88%),separatively.There were no differences among high,low,and none replicated status (P>0.05).HBVDNA particles were mainly located in the cytoplasm,intercellular capillary vessels of the thymus tissue of the fetus,fewer in the nuclei.Conclusions:There existed replication in the thymus tissue of the died fetus.There was no association between HBV replicated status in the pregnant women and the expression of HBVDNA in the thymus tissue of the died fetus.
出处
《实用妇产科杂志》
CAS
CSCD
北大核心
2003年第2期107-108,共2页
Journal of Practical Obstetrics and Gynecology
基金
铁道部 2 0 0 0年自然科技资金资助项目 (J2 0 0 0Z0 95)
