摘要
RNA干涉 (RNAinterference ,RNAi)是 1998年首次在秀丽线虫中发现并证明属于转录后水平的基因沉默 (PT GS)机制。它利用双链RNA(dsRNA)特异性地降解相应序列的mRNA成为siRNA ,从而特异性地阻断相应基因的表达 ,广泛存在于从真菌到植物、从无脊椎动物到哺乳动物的各种生物中。本文介绍了基因沉默的机理、RNA干涉的分子机制及技术应用等方面的进展 。
RNA interference(RNAi) is first discovered in C.elegans in 1998 and was identified to be involved in Post transcriptional gene silencing. It degrades mRNA specifically into siRNA through the mediation of corresponding double stranded RNA, leading to the suppression of specific gene expression. Also, RNAi exists in many species from eukayotic organisams such as fungi to plant, from invertebraet to mammalian. In this paper, recent advances of the molecular mechanism of gene silencing and RNAi, the RNAi technique and it's application were reviewed. The discovery of RNAi and the following studies on the molecular mechanism lead to the development of new strategies for blocking gene function and may yield RNA based genotherapy for human disease.
出处
《基础医学与临床》
CSCD
北大核心
2003年第1期24-29,共6页
Basic and Clinical Medicine
