摘要
目的 :探讨糖尿病肾脏损伤的早期诊断方法。方法 :采用免疫透射比浊法检测尿微量白蛋白 (mALB)和尿β2 -微球蛋白 (β2 -MG) ,终点比色法测尿N -乙酰 - β -D -氨基葡萄糖苷酶 (NAG) ,Jaffe速率法测尿肌酐 (CRE)。结果 :糖尿病患者尿mALB/CRE、β2 -MG/CRE及NAG/CRE值较正常对照组增高 (P <0 .0 1)。三项指标联合检测在糖尿病中阳性率可达4 8.78% ,而尿常规蛋白定性检测阳性率仅为 17.0 7%。结论 :联合检测尿mALB、β2 -MG及NAG是糖尿病肾脏损伤早期诊断灵敏、可靠的实验室指标。
Objective:To study diagnostic manner of early renal damage for diabetes. Methods:Urinary microalbumin and β 2-microglobulin were determined with immunoturbidimetry, Urinary N-acetyl-β-D-glucosaminidase(NAG) was determined with endpoint Colorimetric analysis. Results:The levels of urinary mocroalbumin?β 2-microglobulin and NAG in diabetes group were higher than those in the control guoup(P<0.01). The positive rete of the three determined items together would go up to 48.78% in diabetes, but, The ordinary positive rate of urinary albumin was only 17.07%. Conclusion:The method is sensitive and reliable to determine Urinary Microalbumin β 2-microglobulin and NAG together for diagnose of early renal damage. [
