摘要
目的 :本研究拟从人混合血浆中提取MBL并初步鉴定 ,为进一步深入研究MBL的一些特性奠定基础。方法 :取混合血浆通过前处理后经过Mannan -Agarose亲和层析的方法纯化 ,收集含蛋白的洗脱液 ,充分透析、浓缩即得 ,用SDS -PAGE对提取物质进行鉴定。结果 :用纯化后的产物经过SDS -PAGE ,在 30kDa处显示两条谱带 (对应的分子量约为 2 8kDa和32kDa处 ) ,回收率为 2 0 %。结论 :成功用二步亲和层析法从健康人血浆中提纯MBL ,取得了比较满意的结果。
Objective:To extract and purify MBL from the mixed human plasma,and idenfify its charccteristic primarily. Methods: First ,the mixed human plasma was collected and pre-treated .then MBL was purified from the treated solution by Mannan-agrose affinity chromatography.The eluent which contained MBL protein was collected and dialysed completely and concentrated? SDS-PAGE was used to identify the purified product. Results:SDS-PAGE showed two bands in the positoion of 30kD(respectively,28kD and 32kD),recovery rate was 20%.Conclusion:It was successful to purify MBL from health human plasma with two-step affinity chromatography. [
基金
河北省自然基金的资助 (项目号为 30 0 377)
河北省卫生厅的资助 (项目号为 980 1 2 )
关键词
MBL
亲和层析
纯化
鉴定
MBL, affinity chromatography, Purification, Identification
