摘要
目的:研究C-jun原癌基因在大鼠变应性鼻炎鼻粘膜的表达,探讨其在变应性鼻炎发病机制中的作用。方法:以卵清蛋白为致敏原建立大鼠变应性鼻炎模型,并于模型成功后30min,4h、8h,取大鼠鼻粘膜组织,应用免疫组织化学技术、计算机图像分析技术、进行粘膜的组织学检查方法,检查C-jun在鼻粘膜组织的表达。结果:激发后30min,C-jun在鼻粘膜固有层中炎症细胞内大量表达,与对照组相比,有明显差异性(P<0.01),激发4h,C-jun在炎症细胞内表达减弱,但与对照组相比,仍有差异性(P<0.05),激发8h,C-jun的表达与对照组相比,无明显差异。结论:原癌基因C-jun的表达可能是变应性鼻炎中炎症细胞活化的早期标志,与炎症细胞的增殖、分化有着密切联系。
Objective:To study the expression of oncogene C-jun in rat nasal mucosa with allergic rhinitis and the significance of C-jun in the mechanism of allergic rhinitis. Methods:Animal model of allergic rhinitis was made by using EA. Immunohistochemical technique, computer imange, analysis technique and histologic examination were used to investigate the expression of C-jun in rat nasal mucosa at 30min,4h and 8h. Results:There was a lot of C-jun protein at 30min. The difference of C-jun between 30min group and control group was significant (P<0.01). The C-jun protein decreased at 4h ,but there was still significant difference (P<0.05). The C-jun was as same as control group at 8h and no difference between them. Conclusion:The expression of oncogene C-jun in inflammatory cells may be a marker in early stage of activation of inflammatory cells,and relate to the producing and secreting of cytokines and inflammatony mediators in inflammatory cells of allergic rhinitis.
出处
《耳鼻咽喉（头颈外科）》
2003年第1期46-48,W004,共4页
Chinese Arch Otolaryngology-Head Neck Surg
关键词
变应性鼻炎
大鼠
基因
(Rhinitis,allergic) (Rats) (Genes)
