摘要
目的 观察胎儿与成人正常皮肤中的表皮细胞生长因子受体 (EGFR)与成纤维细胞生长因子受体 (FGFR)在细胞膜上表达的差异 ,探讨胎儿无瘢痕形成的分子机制。方法 对妇产科意外流产的不同妊娠期胎儿和整形外科手术过程中作为供皮区的成人正常皮肤标本 ,应用免疫组织化学技术观察EGFR与FGFR 2的变化。结果 妊娠胎儿的皮肤内EGFR和FGFR 2染色阳性的表皮细胞较多 ,贯穿于全层上皮 ,而真皮中成纤维细胞呈阳性标记的极少。随胎龄的增加 ,两受体的表达增加 ;成年人正常皮肤中EGFR多集中在表皮细胞的基底层 ,两种生长因子受体抗体阳性染色的细胞明显多于胎儿皮肤。结论 胎儿和成年人的皮肤中 ,EGFR染色与FGFR 2表达的差异 ,可能是造成胎儿无瘢痕愈合的重要因素之一。
Objective To observe the expression characteristics of EGFR and FGFR-2 in normal skin from fetal and adult, and attempted to probe the molecule mechanism of fetal scarless healing. Methods The skin samples of fetal and adult were taken from abortive fetus of obstetrics unit and donor site of plastic operation patients in our burn unit, respectively. EGFR and FGFR-2 were used as the biochemical markers for reparative cells. Immunohistochemistry staining technique was employed to determine the expressive levels of different epithelial cells markers. Results There were EGFR and FGFR-2 antibody positive cells in normal skin from fetal and adult, but the expressive levels of EGFR and FGFR-2 protein had apparent difference, with the time of fetation increasing, the EGFR and FGFR-2 positive expression rate became stronger gradually. The number of FGFR-2 antibody positive cells found in adult skin was much more than that in fetal skin. Conclusion There were the inherent differences of EGFR and FGFR-2 antibody immunohistochemistry staining in cells of adult and fetal skin.which may be an essential facet of fetal scarless healing.
出处
《中华整形外科杂志》
CAS
CSCD
北大核心
2003年第2期91-94,共4页
Chinese Journal of Plastic Surgery
基金
国家重大基础研究规划资助项目 (G19990 5 42 0 4)
国家自然科学基金资助项目 ( 30 170 96 6 )
国家杰出青年科学基金资助项目 ( 395 2 5 0 2 4)
博士后基金 (中博基 [2 0 0 1]No .14 )
