摘要
目的 了解线粒体烟酰胺腺嘌呤脱氧核苷酸 (NADH)脱氢酶第一亚单位 (ND1)基因中3 3 94位点T→C突变与我国老年人 2型糖尿病的关系。 方法 收集无血缘关系的 2 0 8例老年 2型糖尿病患者 ,同时以 180例无糖尿病家族史的老年糖耐量正常者作为对照 ,用聚合酶链反应扩增、限制性内切酶HaeⅢ消化进行点突变筛选。 结果 发现 9例患者存在线粒体DNAND1基因 3 3 94位点T→C突变 (4 3 % ) ,非糖尿病者中仅有 1例突变 (0 6% ) ,两组比较差异有显著性 (P <0 0 5 )。 结论 线粒体DNAND1基因中 3 3 94位点T→C突变可能与我国老年人
Objective To investigate the relationship between point mutation of nt3394 T→C of mitochondrial DNA ND1 and type 2 diabetes mellitus(DM) in the elderly. Methods Two hundred and eight patients with type 2 diabetes mellitus and 180 subjects with normal glucose tolerance without DM family history were included. The mutation was determined by polymerase chain reaction and restriction fragment length polymorphism(PCR RFLP). Results The point mutation of nt3394 T→C of mitochondrial DNA ND1 was found in 9 of 208 elderly patients with type 2 DM(4 3%), and 1 of 180 controls (0 6%). The incidence of this mutation was significantly different between the two groups ( P <0 05). Conclusions The point mutation of nt3394 T→C in mitochondrial DNA ND1 may not be an independent factor to type 2 DM in the elderly.
出处
《中华老年医学杂志》
CAS
CSCD
北大核心
2003年第3期133-135,共3页
Chinese Journal of Geriatrics
