摘要
目的 :调查幽门螺杆菌 (Hp)几种表面蛋白体外对T细胞增殖的影响和在小鼠体内的免疫保护作用。方法 :评价Hp全菌抗原、尿原酶 (Urease)、黏附素 (hpaA)、外膜蛋白 2 5(Hop2 5)和 38(Hop38)对人外周血T细胞及小鼠CD4 + T细胞增殖的影响 ;与佐剂合用 ,评价上述重组蛋白对小鼠Hp感染的免疫预防作用。结果 :Urease和Hop2 5可刺激人及鼠T细胞增殖 ,hpaA只能刺激Hp+ PBL增殖 ,而Hop38则有毒性作用 ;Hop2 5和Hop38均可产生 60 %的完全保护 ,hpaA可产生 1 0 0 %的部分保护即降低细菌定植密度 ,而Urease只能产生 40 %的部分保护。结论 :外膜蛋白可能是一组高效的Hp疫苗免疫原 ;其长期免疫效果及对T细胞功能的活体调节作用尚需进一步评价。
Objective:To evaluate the effect of several surface proteins of Helicobacter pylori(H pylori) on the proliferation of peripheral blood lymphocytes(PBL) and CD 4 + spleenocytes of mice and to determine their effect to protect H pylori infection in mice via immunization.Methods:PBL of H pylori infectious and unifectious patients were isolated and CD 4 + spleenocytes were isolated from mice.Their proliferation in response to the surface proteins of H pylori such as recombinant urease,hpaA,and outmembrane protein(Hop)25 and 38 were determined by the 3 H thymidine incorporation assay.Moreover,The colonization of H pylori in mice was evaluated after the immunization with these proteins as well as mucosal adjuvant,cholera toxin.Results:Urease and Hop25 were capable of inducing T cell proliferation,while hpaA can only induce the proliferation of T cell isolated from H pylori infectious subjects.The proliferation of both human and mouse T cells was inhibitied by Hop38.In regard to the immunization,Hop25 and 38 protected 60% fo the mice from infection.hpaA decreased the colonization of H pylori in all of the animals,whereas urease only generated 40% of partial protection.Conclusion:Outmenbrane protein of H pylori seems to be good immunogen candidates in the vaccine preparation.The long term effect of immunization and their effect in regulating T cell function in vivo remain to be studied.
出处
《中国微生态学杂志》
CAS
CSCD
2003年第1期4-6,共3页
Chinese Journal of Microecology
基金
国家自然科学基金资助课题编号 30 170 890
关键词
幽门螺杆菌
外膜蛋白
疫苗
Helicobacter pylori
Outmembrane protein
Vaccine
