摘要
应用生物信息学方法 ,对已完成测序的 76种细菌基因组进行比较 ,分析细菌基因组中编码区及密码子上碱基使用频率情况。结果显示 :1 先导链与滞后链上在编码区的碱基使用频率无明显差异且显著正相关。 2 先导链与滞后链在第一、第二、第三密码子碱基使用频率基本一致且显著正相关。结果表明 ,选择压力及自然突变对DNA双链总体碱基分布的影响相等。
During the Evolution,effected by select pressure or nature mutation,the compositons of bacteria genomes is various.And many experiences prove that the genes between leading strand and lagging strand is disticntly in copy,transcription and repair.Some scholars presume that the bases distribution is difference between the two strand,to verify the guess,we using the technology of bioinformatics,compare the base usage between the DNA double strand in 17 species bacteria.The result show:1 there is same bases usage frequency in coding sequence between leading strand and lagging strand 2 There also same bases usage frequency in first codon,second codon and third codon.It suggest that there is a equilibrium between the two strand by the effect of select pressure and nature mutation.
基金
国家自然科学重点基金
中山大学青年教师科研启动基金 (3 3 110 113 10 91)资助(No.6993 5 0 2 0 )~~
