摘要
端粒酶的激活及其调控机制至今仍不清楚。为研究喉癌发生中端粒酶表达规律及激活的可能机制 ,我们克隆了hTERTcDNA片段并制备了抗hTERT单克隆抗体。应用此抗体对喉癌组织进行了免疫组化检测 ,发现喉癌分化程度降低与癌组织中hTERT阳性细胞率增高有关 ;而c Myc表达与hTERT表达呈明显正相关 ,提示c Myc可能对喉癌发生过程中端粒酶的激活起着重要作用。这些研究表明 ,喉癌的发生可能是由于c Myc的过度表达使端粒酶表达上调 ,从而使喉鳞状上皮细胞达到永生化 ,这一机制不仅存在于喉癌发生的早期 。
The mechanism of telomerase activation is still not clear till now.In order to understand the expressional mode of telomerase and the mechanism of telomerase activation in laryngeal carcinogenesis,we cloned a fragment of hTERT cDNA and prepared a monoclonal antibody against hTERT.We performed immuno histochemical staining in laryngeal cancer tissues using this antibody.We found that the frequencies of hTERT positive cells were positively correlated with undifferentiation of cancer tissues and that the expression of hTERT was positively correlated with levels of c Myc,which suggested that c Myc might play an important role in activation of telomerase.These results revealed that overexpression of c Myc upregulated telomerase,which in turn resulted in immortalization of laryngeal squamous cells,and this mechanism existed not only in the initiation of laryngeal carcinogenesis but also in the whole process of cancer development.
基金
国家自然科学基金 (3 0 1710 0 8)
卫生部科学研究基金 (981198)项目资助~~
