摘要
目的 探讨高原环境对人体免疫功能的影响。方法 选择18-22周岁健康男性75人,在他们从平原急进海拔2300 m的高原地区48 h和在该地区生活适应50 d后,分别采集血液标本,用ELISA法测定可溶性白细胞介素-2受体(sIL-2R)和人生长素(HGH),用免疫扩散法测定免疫球蛋白(IgG、IgA、IgM)和补体(C3、C4),循环免疫复合物(CIC)用聚乙二醇法测定。结果 进入高原50 d后血液中sIL-2R和IgG含量比进入高原48 h显著下降(P<0.01);CIC和IgA的含量比进入高原48 h显著增加(P<0.01);进入高原48 h和50 d IgM、C3、C4和HGH差异无显著性(P>0.05)。结论 人体急进高原后,首先出现细胞免疫抑制和IgG的应激性变化,随着对高原环境的逐渐适应,细胞免疫逐渐得到恢复,分泌型免疫球蛋白IgA的合成分泌增加,血液中的CIC含量也升高。
Objective To explore the effects of plateau environment on soluble interleukine-2 receptor (sIL-2R) and humoral immunity of people rapidly entering plateau. Methods The blood samples were collected from 75 healthy males, aged 18-22 years at the 48th hour after they rapidly entering plateau with altitude of 2 300 m from plain and at the 50th day after they having adapted themselves to the plateau environment. The contents of sIL-2R and human growth hormone (HGH) were determined by ELISA method, the contents of immunoglobulins (IgG, IgA, IgM) and complements (C3, C4) were determined by immunodiffusion method, the contents of circulatory immune complexes (CIC) were determined by polyethylene glycol method. Results The blood samples showed significantly lower contents of sIL-2R and IgG and significantly higher contents of CIC and IgA (P<0.01) collected from the 75 subjects at the 50th day after entering plateau compared with those at the 48th hour after entering plateau, but the contents of IgM, C3, C4 and HGH in blood samples collected in the above mentioned corresponding periods from 75 subjects showed no significant differences (P>0.05). Conclusion The cellular immunosuppression and irritable changes of IgG occured first with the prolongation of adaption periods to the plateau environment, the inhibited cellular immunity was restored gradually, the synthesis and secreation of SIgA as well as the content of CIC in blood increased.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2003年第2期96-97,共2页
Journal of Environment and Health
