摘要
对用聚丙烯酰胺凝胶,制备的固定化多核苷酸磷酸化酶与游离酶的特性比较表明:在以CDP为底物时,游离酶的km=6.4×10^(-3)M,而固定化酶的km(表观)=9.14×10^(-3)M,比游离酶增大,固定化酶的最适温度范围扩宽,稳定性提高。
The characteristics of Immobilized polynucleotide which is made up of polyaerylamidegel is compaed with that of the free enzyme. The result shows that when CDP is usud as basic material, the free enzyme 's km is equal to 6.4×10^(-3)m while the immobilized enzyme is equal to 9.14×10^(-3)m which is larger than that of enzyme. The proper temperature range of immobilized enzyme has been widened and its stabilized enzyme has been improved.
关键词
聚丙烯酰胺
凝胶
固定化
PNPASE
polyaerylamide gel
immobilized
polynucleotide phosphorylase
