摘要
用低强度瞬态电磁场对家猪的精子细胞进行电穿孔处理 ,比较了精子细胞电穿孔前后的形态变化 .发现台盼蓝染料能够进入电穿孔后的家猪精子细胞 ,通过扫描电镜观察到电穿孔后的细胞膜表面有明显的孔洞 .实验结果表明 ,低强度瞬态电磁场能够促使猪精子细胞穿孔 ,并能促使外源物质进入细胞内部 .
We treated the sperms of domestic pig with weak electro-magnetic pulse field and compared the shape change of the sperms.we observed that Trypan Blue can enter the sperm cells and there are obvious holes in the cell surface by scanning electron microscope. It was confirmed that weak electro-magnetic pulse field can cause electroporation of domestic pig sperms and make the little exogenous molecules enter the sperm cells.
出处
《深圳大学学报（理工版）》
EI
CAS
2003年第1期36-39,共4页
Journal of Shenzhen University(Science and Engineering)
基金
国家自然科学基金资助项目 (60 0 71 0 2 4 )
关键词
电穿孔效应
低强度瞬态电磁场
家猪
精子
扫描电镜
细胞
weak electro-magnetic pulse field
pig sperm
scanning electron microscope
electroporation
