摘要
目的 研究睾丸扭转复位后的组织结构变化及其发生机制。方法 建立大鼠睾丸扭转和复位模型。分别于术后第 3、7d观察扭转侧睾丸的变化 ,测定睾丸超氧化物歧化酶 (SOD)、过氧化氢酶 (CAT)、谷胱甘肽过氧化物酶 (GSHPx)活性以及丙二醛 (MDA)含量。结果 睾丸扭转复位后其损伤进行性加重。复位后第 3d ,与对照组相比 ,扭转组睾丸SOD、CAT活性显著下降 (P <0 .0 1 ) ,GSHPx活性明显下降 (P <0 .0 5) ,MDA含量显著上升 (P<0 .0 1 ) ;复位后第 7d ,扭转组睾丸重量减轻 (P <0 .0 5) ,SOD/ (CAT +GSHPx)比值明显下降 (P <0 .0 5) ,MDA含量仍明显高于对照组 (P <0 .0 1 )。
Objective To investigate histological changes and mechanisms in experimental torsed/detorsed testes of rats.Methods The model of testicular torsion/detorsion was established in Sprague Dawley male rats.On the third day and seventh day after operation respectively,the activities of superoxide dismutase(SOD),catalase(CAT) and glutathione peroxidase (GSHPx) and the level of malodialdehyde (MDA) were detected by spectrophotom etric method and the histological changes were studied.Results Testicular torsion induced a time dependant pathological damage to ipilateral testis.Compared with that of the control groups,the activities of SOD,CAT and GSHPx all decreased remarkly (P<0.01,P<0.01and P<0.05 respectively) and average MDA level increased significantly (P<0.01) in the torsed testes three days later.On the seventh day after operation,the testes weight and the ratio of SOD/(CAT+GSHPx)decreased predominantly (both P<0.05) and MDA level remained to increase remarkably (P<0.01) as compared to that of the control.Conclusions The decrease of antioxidase activity and reactive oxygen species overproduction play an important role in pathological damage of testicular torsion.
出处
《医学新知》
CAS
2003年第1期17-19,共3页
New Medicine
