摘要
目的观察可被放射线转录激活的早期生长反应基因 1(earlygrowthresponse 1,Egr 1)启动子驱动的单纯疱疹病毒胸苷激酶基因 (herpssimplexvirusthymidinekinase ,tk)对肝癌细胞的高效杀伤作用。方法构建以Egr 1为启动子 ,以tk为目的基因的重组质粒pET ,经脂质体介导转染人肝癌细胞株SMMC 772 1,命名为SMMC/ET细胞 ,经G418抗性筛选、60 Co γ射线照射后 ,加入前药丙氧鸟苷 (ganciclovirGCV) ,测定其对肝癌细胞的杀伤作用。结果与对照组肝癌细胞 ( 0 88± 0 12 )相比 ,经γ射线照射后 ,前药GCV可明显提高对SMMC/ET肝癌细胞的杀伤效率 ( 0 0 7± 0 0 3) (P <0 0 0 1)。结论放射敏感性自杀基因在γ射线作用下可以显著提高对肝癌细胞的杀伤作用。
ObjectiveTo observe the killing effect on hepatocellular carcinoma(HCC) cell lines by growth response-1(Egr-1) promoter activated herps simplex virus thymidine kinase (tk). MethodsPlasmid pET was constructed by fusing of Egr-1 promoter to the upstream of tk gene and transfect human hepatocellular carcinoma cell lines SMMC-7721(SMMC/ET) with lipofectamine as a delivery system. The cloned cells, after selected with G418, and exposure to γ-radiation by a 60Co source, were added with prodrug GCV. The viability of cell lines was observed. ResultsAfter irradiation, transfected cell lines (0.07±0.03) was killed by prodrug GCV at higher percentage compared with control group(0.88±0.12)(P<0.001).The viability was 8% of control group.ConclusionsRadio-sensitized suicide gene activated by γ-ray significantly enhanced killing on HCC.
出处
《中华普通外科杂志》
CSCD
北大核心
2003年第3期143-145,共3页
Chinese Journal of General Surgery
