摘要
目的 观察核心编码因子 (cbfal)基因在人的类成骨细胞株TE85的表达与骨钙素、Ⅰ型胶原和碱性磷酸酶合成及分泌的关系。方法 原位杂交组织化学技术、放射免疫法、硝基苯酚法和3H脯氨酸掺入法。结果 (1)细胞培养至第 3天可见cbfal基因表达 ,第 9天达高峰 ,12d时表达减弱 ;(2 )骨钙素和Ⅰ型胶原的表达在第 12天时达峰值 ,碱性磷酸酶在第 15天时达峰值。结论 cbfal基因表达与成骨细胞标志物表达之间的时差关系 。
Objective To investigate the relationship between the expression of core binding factor alpha 1(Cbfal)and synthesized and secreted osteoblast marker including type 1 collagen,osteocalcin and alkaline phosphatase in human osteoblast like TE85(HOS TE85)osteosarcoma cell line Methods In situ hybridization histochemical techniques,radioimmunoassay, nitrophenol assay and the incorporation and hydroxylation of Proline Results (1)The expression of cbfal gene began to be found find in on the third day of cell culture and peaked in the ninth day,and weakened on the twelfth day (2)Values of type 1 collagen synthesized and osteocalcin secreted in HOS TE85 cell line peaked on the twelfth day, and that of alkaline phyosphatase on the fifteenth day Conclusion Cbfal gene may have regulatory roles in synthesizing and secreting osteoblast markers including type 1 collagen,osteocalcin and alkaline phosphatase in human osteoblast like TE85 cell line
出处
《中国骨质疏松杂志》
CAS
CSCD
2003年第1期16-18,共3页
Chinese Journal of Osteoporosis
基金
陕西省自然科学基金资助项目 (99SM30 )
