摘要
目的 研究孕激素对正常成人成骨细胞转化生长因子 (TGF) β1、TGF β2表达的调节作用 ,探讨孕激素治疗绝经后骨质疏松症 (OP)的作用机制。方法 鉴定成人成骨细胞 (hOB) ,测定碱性磷酸酶 (ALP)活性、骨钙素分泌 ,VanGieson氏苦味酸酸性复红染色法进行Ⅰ型胶原染色。hOB用孕酮干预。Northern杂交、ELISA分别检测hOBTGF β1、TGF β2mRNA和蛋白质分泌。结果 观察到hOB分泌的ALP活性为 (74 3± 4 7)mU/mg蛋白 ,培养上清液骨钙素含量为 (3 84± 0 3 9)ng/ml蛋白 ,Ⅰ型胶原染色呈红色。观察到孕酮增强hOBTGF β1、TGF β2mRNA表达呈剂量依赖性 (P <0 0 0 1) ;10 -9mol/L孕酮诱导 12～ 2 4h促进TGF β1、TGF β2mRNA表达 ,呈时间依从性。孕酮促进hOBTGF β1、TGF β2蛋白质分泌呈剂量依赖性 (P <0 0 0 1) ;10 -9mol/L孕酮诱导 12～ 2 4h促进TGF β1、TGF β2蛋白质分泌 ,呈时间依从性。结论 孕激素可能通过诱导成骨细胞TGF β1、TGF β2表达 ,促进成骨细胞增殖与分化 ,增强成骨功能及骨基质合成 。
Objective To characterize the effects of progesterone (P) on the expression of transforming growth factors beta (TGF β1, β2, and β3) in normal human osteoblasts (hOB) in order to understand the mechanism by which progestin acts on bone Methods Characterization of hOB by ALP activity assay,osteocalcin assay,and type Ⅰ collagen staining Northern blot analysis and ELISA were used to show the actions of P on expression of TGF β1, β2 mRNA and protein production in hOB cell cultures Results Osteoblasts were the primary cell type found in our bone derived cell cultures from donors After 12-24 h of treatment,progesterone at 10 -9 M increased TGF β1,β2 mRNA levels and protein production in cultures of hOB Treatment with increasing dose of progesterone caused a dose dependent increase in the expression of TGF β1,β2 mRNA and protein by hOB Conclusion A direct action of progesterone on osteoblasts may be mediated by up regulated TGF β1,β2 production
出处
《中国骨质疏松杂志》
CAS
CSCD
2003年第1期12-15,共4页
Chinese Journal of Osteoporosis
基金
国家"九五"攻关资助项目(96 90 6 0 5 0 5 )
国家自然科学基金资助项目(39970 34 7)
