摘要
目的 探讨环氧化酶 (Cyclooxygenase,COX) 2基因表达在食管上皮癌变中的作用 ,为食管癌早期诊断及非类固醇类抗炎药 (Nonsteroidalanti inflammatorydrugs ,NSAIDs)在食管癌高发区进行化学预防提供理论依据。 方法 从食管癌高发区人群中采集食管上皮细胞标本 ;并收集该省食管癌高发区食管鳞癌及癌前病变新鲜标本 76例。采用间接免疫荧光标记技术 ,应用流式细胞仪对COX 2及 p5 3的表达进行定量检测。 结果 COX 2在食管脱落上皮细胞中的表达随异型性的增高而逐渐增加 ,在癌细胞组达最高 (FI=1.6 2± 0 .2 3) ;COX 2在高分化鳞癌组表达最高 (FI=2 .37± 0 .71) ,并随癌细胞分化程度的降低而显著减少。p5 3的表达在脱落上皮细胞中随细胞异型性的增高逐渐增多 ,在癌细胞组表达含量最高 (FI =2 .2 8± 0 .2 0 ) ;而在癌组织中的表达 ,则随分化程度的降低继续升高。对COX 2与p5 3表达的相关分析发现 ,从正常上皮至高分化鳞癌两者表达呈显著正相关 (r=0 .42 4,P =0 .0 0 2 ) ;而在不同分化程度癌中的表达 ,两者呈显著负相关 (r =- 0 .345 ,P =0 .0 31)。结论 COX 2及 p5 3表达在食管上皮早期癌变进程中异常增高 ,并有明显的协同作用。COX 2单独或与p5 3联合检测可以作为食管上皮早期癌变的分子标志。
Objective To explore the role of COX-2 and p53 genes in the carcinogenesis of esophageal epithelia, and its use in the early diagnosis and as a chemopreventional indicator of esophageal carcinoma. Methods The samples of esophageal epithelial cells were collected from the population living in the high risk area of esophageal carcinoma, and the specimens of esophageal squamous cell carcinoma (SCC) and its precancerous lesions were collected from the patients after esophagectomy. The proteins of COX-2 and p53 were labeled by indirect immunofluorescence technique and detected by flow cytometry. Results The expression of COX-2 gene increased in the exfoliated epithelial cells of the esophagus as its progression from normal to carcinoma, and the fluorescence index (FI) reached peak in carcinoma cell group (FI=1.62±0.23); The expression of COX-2 was the highest in the well differentiated SCC (FI=2.37±0.71) and then decreased from well to poorly differentiated SCC. The expression of p53 gene was also increased in the exfoliated epithelial cells of the esophagus as its progression to carcinoma, (F1=2.28±0.20) and was the highest in poorly differentiated SCC (FI=2.70±0.82).The overexpressions of COX-2 and p53 had positive correlation ( r=0.424, P =0.002) as the progression from normal epithelia to well differentiated SCC, and they had a negative correlation in different degree of differentiation. Conclusion The expressions of COX-2 and p53 are up-regulated in the early carcinogenesis of esophageal epithelial cells,and shows a positive correlation. COX-2 alone or both COX-2 and p53 may be a good molecular marker for the early diagnosis of esophageal SCC and used as a chemopreventional indicator of esophageal carcinoma in high-risk area.
出处
《肿瘤》
CAS
CSCD
北大核心
2003年第2期111-114,共4页
Tumor
基金
"九五"国家医学科技攻关课题 (编号 :96 -90 6 -0 1-0 2 )
河北省自然科学基金课题 (编号 :30 135 4)
