摘要
目的 探讨胃癌中医证型与p5 3、bcl 2、bax基因蛋白表达的相关性 ,提示病与证之间的内在联系以及证的物质基础。方法 选择进展期低分化胃癌 6 5例 ,辨证分为脾虚组与非脾虚组 ,对其主要症状进行半定量积分 ,并设正常对照组 2 0例。采用免疫组化S P法检测病理组织中p5 3、bcl 2、bax蛋白的表达。结果 脾虚组患者p5 3、bcl 2蛋白的阳性表达率及表达水平显著高于非脾虚组(P <0 0 5 ) ,bax蛋白阳性表达脾虚组低于非脾虚组 ,但组间比较差异无显著性 ;脾虚患者症状积分与p5 3、bcl 2蛋白表达水平呈正相关 (P <0 0 1) ,与bax蛋白表达水平无相关性 ;非脾虚患者症状积分与p5 3、bcl 2、bax蛋白表达水平均无相关性。结论 p5 3、bcl 2蛋白的差异表达与胃癌脾虚证的发病学机理有关 ;p5 3、bcl 2基因是脾虚证的物质基础之一。
Objective To investigate the correlation between TCM syndrome patterns of gastric carcinoma and the expressions of the genetic proteins p53, bcl 2 and bax, for revealing the inner relationship between a disease and a TCM syndrome and finding the material basis of a TCM syndrome. Methods 65 Cases of poorly differentiated gastric carcinoma at the progressive stage were chosen and divided into the group of splenic deficiency (SD) and the group of non splenic deficiency (NSD) on the basis of TCM syndrome differentiation, and their main symptoms were processed by a semi quantitative scoring method; the normal control group was made up by 20 subjects. The expressions of the genetic proteins p53, bcl 2 and bax in the pathologic tissues were detected by the S P method. Results The rates and the levels of the positive expressions of p53 and bcl 2 were significantly higher in the patients in SD group than those in NSD group ( P <0 05); the level of the positive expression of bax was lower in SD group than that in NSD group but there was no significant difference between the two results; there was a positive correlation between the values of the symptomatic scoring and the levels of the expressions of p53 and bcl 2 in SD patients ( P <0 01), but there was no such correlation between the values of the symptomatic scoring and the level of bax expression; and there was also no correlation between the values of the symptomatic scoring and the levels of the expressions of p53, bcl 2 and bax in NSD patients. Conclusion The discrepant expressions of p53 and bcl 2 found in this experiment are related to the pathogenesis of SD syndrome of gastric carcinoma, and p53 and bcl 2 genes are part of the material basis of SD syndrome.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2003年第2期56-59,共4页
Journal of Beijing University of Traditional Chinese Medicine
基金
陕西省中医药管理局基金资助课题 (No .990 0 3 )
