摘要
从广西部分猪场血痢病猪中,取血粪及结肠括取物接种于培养基,在一个大气压的80%H,和20%CO_2,并加有催化剂钯的环境中培养,分离出2株猪痢疾密螺旋体样微生物。根据其典型的形态、β-溶血型、具有致病性等特点,鉴定为致病性的猪痢疾密螺旋体(代号为 ThB_1、ThB_2),从而在病原学上确诊了广西的猪痢疾。作者还进一步对广西部分猪场进行了该病的流行病学调查;对分离的病原株进行了电镜形态学观察、保存期试验、微量凝集试验(MAT)和 SDS—聚丙烯酰铵凝胶电泳试验(SDS-PAGE)等研究。
Feces and colonic samples collected from pigs which suffered from“blood dysentery”in some pigfarms of Guangxi were cultured on the medium containing the catalyst,palladium.The cultures were incubated under conditions of atmosphere,80% H_2,and 20% CO_2.Two strains isolated were suspected to be Treponema hyodysenteria.According to their typical morphology,β-hemolysis,and pathogenicity,they were identified as the pathogen of swine dysentery(ThB_1 and ThB_2).The swine dysentery was determined etiologically and investigated epidemiologically.Strains isolated were analysed by electron-microscopic observation,preservation period test,microagglutination test,and SDS-PAGE.
关键词
猪
痢疾
诊断
病原
广西
swine dysentery
epidemiology
Treponema hyodysenteria
isolation and identification
preservation period
microagglutination test
SDS-PAGE
