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包覆包醛氧淀粉固定脲酶制备及对尿素吸附 被引量:12

Preparation of Encapsulated Urease-Immobilized Aldehyde-Coated Oxystarch and Its Absorption for Urea
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摘要 包醛氧淀粉固定脲酶作为口服吸附剂,经口服首先进入胃中的酸性环境,使脲酶失活.为保护脲酶的活性,以海藻酸为主要原料,在固定脲酶表面覆上一层海藻酸膜,制成包覆型包醛氧淀粉固定脲酶,并对包覆的工艺和配方进行了研究.实验结果表明,经过包覆的包醛氧淀粉固定脲酶样品在经过0.1mol L-1的HCl溶液浸泡3h后,其对尿素的吸附量仍可达15mg g,吸附量明显优于包醛氧淀粉,是一种高效的尿素吸附剂. When the ureaseimmobilized coated dialdehydestarch as an oral absorbent for urea comes into the stomach firstly,the ureaseimmobilized loses its activity in acidic environment of stomach.To protect the activity of ureaseimmobilized, this paper presents the preparation of encapsulated ureaseimmobilized coated dialdehyde starch by using alginate as the main raw material. The appropriate consistence and process of this sample are also studied. Experimental results demonstrate that after dipping the sample into 0.1mol/L HCl solution for 3 hours, its absorbing capacity for urea reaches 15 mg/g. The absorbing capacity for urea of it is better than that of coated dialdehyde starch. Therefore, it is a kind of high effective absorbent for urea.
机构地区 天津大学理学院
出处 《天津大学学报(自然科学与工程技术版)》 EI CAS CSCD 北大核心 2003年第2期197-200,共4页 Journal of Tianjin University:Science and Technology
关键词 脲酶 包醛氧淀粉 海藻酸 壳聚糖 urease coated dialdehyde-starch alginate chitosan
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参考文献4

  • 1陈炎泉 于九皋.覆醛氧化淀粉的研究[J].天津大学学报,1987,(2):13-21.
  • 2Yu Jiugao, Tian Ruchuan, Li Xinrui. A study of starch as a supporter in the enzyme immobilization[J]. Starch/Starke,1994,46(2):49-51.
  • 3张晓东,龙世农,刘景林,于九皋.包醛酶淀粉吸附性能的研究[J].化学工业与工程,1996,13(3):11-14. 被引量:6
  • 4吉尔鲍特G G. 酶法分析手册[M]. 缪兴全, 陈石根译.上海:上海科学技术出版社, 1983.

二级参考文献1

  • 1陈炎泉,段梦林,于九皋,田汝川.尿素氮吸附剂—覆醛氧化淀粉的研究[J]天津大学学报,1987(02).

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