摘要
本研究采用6 0 Coγ射线照射中国仓鼠肺成纤维细胞 (CHL) ,利用单细胞凝胶电泳技术和检测克隆形成率对辐射诱发的基因组不稳定性进行了探讨。将对数生长期的细胞分成不同剂量组 ,6 0 Coγ射线照射后传代培养 ,在 3 3代后各剂量组再次统一照射 2Gy,进行辐射损伤的检测。结果表明 ,首次照射剂量与子代二次照射后的损伤程度存在剂量效应关系。本次实验结果说明 ,6 0 Coγ射线不仅在CHL细胞中产生直接的生物效应 。
In this paper, the China hamster lung fibroblasts (CHL) cell′s genomic instability induced by 60 Co γ rays was studied by the methods of single cell gel electrophoresis (SCGE) and cloning efficiency. The fast growing cells were grouped and then irradiated by 60 Co γ rays. After 33 population doubling, the progenies were secondly irradiated with 2 Gy 60 Co γ rays. The results showed that the second damage was correlated with the original irradiation dose. It can be concluded that the 60 Co γ rays can not only induce the immediate biological effects in CHL cells, but also can lead to the genomic instability in the descendants.(
出处
《辐射防护》
CAS
CSCD
北大核心
2003年第2期119-121,共3页
Radiation Protection
