摘要
AIM:To study the effect of cerebral ischemia-reperfusion(CI /RP)injury on the function and cycle of T cells in ra t.METHODS:Using animal model of brain ischemia-reperfusion injury,H-TdR incorporation and flow cytom eter to detect the change of T cell function a nd cell cycle.RESULTS:After CI /RP,the proliferation ability,clon e formation rate and IL-2production ability of T cells of experimental group were apparently lower than those of contr ol group(P<0.01),and it more apparent with prolonged ischemia time;with CI /RP the recovery of 3-hour gro up was more rapid than that of 6-hour group;after CI /RP injury,the proliferation cycle of T cells in each grou ps changed,the cells in G 0 /G 1 stage were more than those of control group(P<0.01);while the cells in S +G 2 +M stages were few than those of contr ol group(P<0.01);when CI /RP,amount of cells in G 0 /G 1 stage decrease gradually and that of cells in S +G 2 +M stages increase gradual-ly.CONCLUSION:after CI /RP,the function of rat sple en T cells decrease and the proliferation and different iation of cells were inhibited.Early-stage reperfusion can recover damaged immune function as soon as possible.
AIM:To study the effect of cerebral ischemia-reperfusion(CI /RP)injury on the function and cycle of T cells in ra t.METHODS:Using animal model of brain ischemia-reperfusion injury,H-TdR incorporation and flow cytom eter to detect the change of T cell function a nd cell cycle.RESULTS:After CI /RP,the proliferation ability,clon e formation rate and IL-2production ability of T cells of experimental group were apparently lower than those of contr ol group(P<0.01),and it more apparent with prolonged ischemia time;with CI /RP the recovery of 3-hour gro up was more rapid than that of 6-hour group;after CI /RP injury,the proliferation cycle of T cells in each grou ps changed,the cells in G 0 /G 1 stage were more than those of control group(P<0.01);while the cells in S +G 2 +M stages were few than those of contr ol group(P<0.01);when CI /RP,amount of cells in G 0 /G 1 stage decrease gradually and that of cells in S +G 2 +M stages increase gradual-ly.CONCLUSION:after CI /RP,the function of rat sple en T cells decrease and the proliferation and different iation of cells were inhibited.Early-stage reperfusion can recover damaged immune function as soon as possible.
出处
《中国临床康复》
CSCD
2003年第3期504-505,共2页
Chinese Journal of Clinical Rehabilitation
关键词
脑缺血再灌注损伤
大鼠
T细胞功能
细胞周期
影响
SUBJECT WORDS:cerebral ischemia
reperfusion inj ury
T-lymphocyte
cell cycle
