摘要
采用离子交换层析和凝胶层析方法 ,从杏鲍菇干样中分离得到多个蛋白组分 ,经枯斑寄主检测 ,发现多个蛋白组分都有抗烟草花叶病毒 (TMV)的活性 ,对TMV的抑制率均在 70 %以上 ,高者可达 99%。其中xb68Ab已得到了纯化 ,分子量约为 2 3 7kD ,在心叶烟和苋色藜上它对TMV侵染的抑制率分别达到 99 43%和 98 9%。
Proteins were obtained by ion |exchange chromatography and gel filtration from the dry sample of %Pleurotus eryngii. % In terms of detection on local lesion host, these components were confirmed to be anti |TMV and their inhibition rates reach more than 70%, or even 99%. The protein named xb68Ab of MW 23 ^7kD was purified by ion |exchange chromatography and gel filtration. its inhibition rate against TMV are 99 ^43% and 98 ^9% on %Nicotiana glutionosa % and %Chenopodium amaranticolor %, respectively.
出处
《微生物学报》
CAS
CSCD
北大核心
2003年第1期29-34,共6页
Acta Microbiologica Sinica
基金
福建省科技厅重点项目 ( 99-Z1- 93)
