摘要
以国际上通用的栽培大豆品种Lee68(耐盐性较强)和非盐生野大豆N23232种群(耐盐性较弱)为参照材料,研究了在低盐(150mmol·L-1NaCl)和高盐(300mmol·L-1NaCl)胁迫下,盐生野大豆BB52种群幼苗体内的O-·2水平和MDA含量,活性氧清除酶系统中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)等酶活性,以及抗坏血酸(AsA)、类胡萝卜素(Car)、谷胱甘肽(GSH)等活性氧清除物质含量的变化。结果表明,在150和300mmol·L-1NaCl胁迫2d时,盐生野大豆BB52种群幼苗根和叶中的O-·2产生速率下降,SOD活性上升,其中叶片表现明显,且叶中APX活性也有增加。在不同浓度NaCl胁迫下,根中MDA含量均未见明显上升,叶片中略有增加。叶中AsA、Car和GSH含量均上升,且升幅相对较大。
In contrast with the annual Glycine max(Lee68,the salttolerant) and Glycine soja (N23232,the saltsensitive),the changes in producing rate of O-·2,contents of MDA,ascorbic acid(AsA),carotenoid(Car) and glutathione(GSH),the activities of superoxide dismutase (SOD),catalase(CAT),peroxidase(POD) and ascorbic acid peroxidase(APX)in halophyte BB52 (Glycine soja) seedlings were investigated under 150 and 300 mmol·L-1 NaCl stresses for 2 d.The results showed that,the producing rate of O-·2 in roots and leaves of BB52 seedlings all decreased,the SOD and the leaf APX activities all increased,as compared to the control plants.Under different concentrations of NaCl,the MDA content in roots showed no obvious ascent,and that in leaves increased slightly.The contents of AsA,Car and GSH in leaf of BB52,N23232 and Lee68 seedlings were all increased,and the increases in BB52 were higher.
出处
《西北植物学报》
CAS
CSCD
2003年第1期18-22,共5页
Acta Botanica Boreali-Occidentalia Sinica
基金
瑞典国际科学基金(IFS)(C/3143-1)
高等学校博士学科点专项基金(1999002005)
国家自然科学基金资助项目(39870069)
关键词
盐胁迫
一年生盐生野大豆
幼苗
活性氧代谢
salt stress
salt-born Glycine soja
producing rate of O_2^-·,contents of MDA
scavenging enzymes of active oxygen
scavenging substances of active oxygen
