摘要
对袋鼠花 (Anigozanthos)组织培养研究表明 :外植体消毒以 0 .1%升汞溶液浸泡 ,7～ 8min为宜 ;不同浓度激素对其愈伤组织形成、分化以及根的形成有不同的影响 ,随着 6 BA浓度的增加 ,愈伤组织的诱导率有增加的趋势 ;生长素与分裂素的比例决定着愈伤组织的分化 ;小苗在 1/ 2MS培养基上生根最好 ,6 BA对其生根有抑制作用。各培养阶段最适培养基 :(1)愈伤 ,MS +6 BA 1mg/L +NAA 0 .1mg/L +3%活性炭 ;(2 )芽诱导 ,MS +6 BA 0 .5mg/L +NAA 0 .5mg/L ;(3)生根 ,1/ 2MS +IBA 0 .2mg/L。
The results of current study show that the optimum sterilization of explants is 7-8 min with 0.1% HgCl 2.The concentration of NAA,6 BA affects the formation of callus,shoot induction and rooting of plantlets.A increase in 6 BA concentration resulted in increased callus formation.The BA/NAA ratio determined the shoot induction.Half strength MS medium proved best for the rooting plantlets and the addition of 6 BA inhibited it.The best media for different cultural stages are:(1)callus:MS+6 BA 1mg/L+NAA 0.1mg/L+charcoal 3%;(2)shoot induction:MS+ 6 BA 0.5mg/L+NAA 0.5mg/L; (3)rooting:1/2 MS+IBA 0.2mg/L.
出处
《园艺学报》
CAS
CSCD
北大核心
2003年第1期113-114,共2页
Acta Horticulturae Sinica
