摘要
目的 :检测白血病患者WT1基因的表达水平 ,并探讨其与临床的关系。方法 :应用逆转录 聚合酶链反应(RT PCR)技术 ,检测 5 8例初治急性白血病 (AL)、2 0例慢性粒细胞白血病 (CML)和 10例非血液病患者WT1基因的表达。结果 :10例非血液病患者WT1基因的表达均呈阴性。 5 8例AL患者 44例检测有WT1基因的表达 ,阳性率为 75 .9% ,其中急性髓性白血病 (AML)阳性率为 86 .4% ,急性淋巴细胞白血病 (ALL)为 42 .9% ,两者差异有显著性 (P <0 .0 1) :AML治疗前WT1的表达水平与完全缓解 (CR)率无相关 (P >0 .0 5 ) ,但倾向于WT1基因低表达者有更高的CR率 (低表达组CR率为 75 .0 % ,高表达组率为 34 .8% )。CML慢性期WT1的表达水平极低 ,随临床分期进展水平增高 ,高表达者预示急变。结论 :AL和CML急变期患者WT1基因过度表达 ,初治AML治疗前WT1的表达水平与疗效无相关 ,但低表达组CR为 75 % ,高表达组CR为 34 .8%。
Objective:To investigate the WT1 expression and evaluate its clinical significance in leukemia.Methods: Fresh bone marrow or peripheral blood cells of 58 patients newly diagnosed of AL,20 with CML,and 10 without-blood disease were studied for WT1 expression.WT1-reverse transcription-polymerase reaction (RT-PCR) was performed.Results:The WT1 expression of 10 none-blood disease patients were all negative.WT1 mRNA was detectable in 44 of 58 AL patients.The positive percentage of WT1 expression was 86.4% and 42.9% in 44 AML and 14 ALL patients,respectively.The difference was significant(P<0.01).There was no statistically significant association between the level of WT1 expression and the achievement of CR(P> 0.05),but a trend to higher CR rates in low group with CR rates of 75.0%,compared to 34.8% in high level group.The level of WT1 in CML increased as the clinical phase progressed and high level might indicate blast crisis.Conclusion:WT1 gene overexpress in AL and blast crisis of CML.The level of WT1 expression was no correlation to CR1 in newly diagnosed AML.but in low group with CR rate of 75.0%,compared to 34.8% in high level group.
出处
《江西医学院学报》
CAS
2002年第5期13-16,共4页
Acta Academiae Medicinae Jiangxi
关键词
白血病
基因表达
WT1
聚合酶链反应
预后
leukemia,gene expression
WT1
polymerase chain reaction
prognosis
