摘要
采用紫外线和氯化锂诱变 ,从甲基对硫磷降解菌假单胞菌 (Pseudomonassp.)DLL -1中获取高效突变株DLL -E1 ,DLL -E2 ,DLL -E3 ,DLL -E4。并对其降解甲基对硫磷性能、降解对硝基苯酚性能、底物广谱性及胞外酶及粗酶液活性等生物学功能进行了研究。与出发菌株相比 ,上述性能均有所提高。选择DLL -E4作为进一步研究的对象 ,详细研究了其对甲基对硫磷及对硝基苯酚的耐受和降解情况。高效菌株的选育为农药残留降解的酶学研究打下了基础。
Summary Four mutants (DLL-E1, DLL-E2, DLL-E3 and DLL-E4) were obtained by UV and LiCl mutation breeding of Methyl Parathion degrading strain Pseudomonas sp . DLL-1. Studies on their abilities to degrade Methyl Parathion and p nitrophenol, substrates, activities of ectoenzyme and crude enzyme showed that all mutants have higher biological activities compared to the original strain. Further studies on resistance and degradation of Methyl Parathion and p nitrophenol by DLL-E4 was conducted.
出处
《土壤学报》
CAS
CSCD
北大核心
2003年第2期293-300,共8页
Acta Pedologica Sinica
基金
农业部"九五"重点项目 (编号 :96环 0 2 -0 2 )资助
