摘要
目的 为研究白藜芦醇 (Res)抗癌及诱导细胞凋亡的机理 ,观察了Res对线粒体通透转变孔道 (PTP)开放的影响。方法 提取大鼠肝线粒体。用氧电极法检测Res对离体线粒体氧化磷酸化的影响 ;通过紫外分光光度仪检测Res作用下线粒体的膨胀 ,借此测定线粒体PTP的开放状态 ;采用荧光分光光度仪测定Res对线粒体膜电位的影响。结果 白藜芦醇抑制线粒体的呼吸 ,降低线粒体的呼吸控制率 ;能剂量依赖性地促进Ca2 + 诱导的鼠肝线粒体PTP开放 ;并且Res可以加速Ca2 + 介导的线粒体膜电位的升高和消失。结论 白藜芦醇诱发细胞凋亡及抗癌的可能途径之一是抑制线粒体的呼吸 ,促进线粒体内Ca2 +
Aim To investigate the mechanisms of anti cancer effect of resveratrol (Res), and the effects of Res in cell apoptosis. The role of Res playing in mitochondrial permeability transition pore (PTP) induction was studied. Methods Mitochondria was prepared from the liver of Wistar rats. The effects of Res on oxygen consumption of isolated mitochondria from rat liver was measured with Clark type electrode and resulted in respiration control rate (RCR); Mitochondrial swelling affected by Res was assessed spectrophotometrically, through the changes in absorbance at 540 nm. The PTP opening was learned from the results. Membrane potential of mitochondia was measured through fluorescence spectrophotometry. Results Res was shown to inhibit the respiration and decrease the RCR of mitochondria. Res can promote the PTP opening mediated by Ca 2+ . Res was shown to promote the increase of mitochondial membrane potential mediated by Ca 2+ and loss of mitochondial membrane potential. Conclusion Res was shown to inhibit mitochondial respiration and induce PTP opening of mitochondria. These may be one of the pathways that Res showed anti cancer action and induce cells apoptosis.
出处
《药学学报》
CAS
CSCD
北大核心
2003年第2期81-84,共4页
Acta Pharmaceutica Sinica
基金
广东省科技计划项目 ( 2KM0 42 0 3S)
