摘要
目的 采用血管内皮细胞 (EC)和平滑肌细胞 (SMC)双层种植方法 ,提高人工血管腔面EC的保存率。 方法 聚四氟乙烯 (PTFE)人工血管经纤维连结蛋白预衬处理 ,管腔面先后种植SMC和EC ,将受试的人工血管接入脉冲式体外灌注装置灌注 1h ,计算比较灌注前后受试标本SMC和EC密度。 结果 灌注 1h后 ,单纯EC种植组 61%细胞脱落 ,单纯SMC种植组 3 6%细胞脱落 ,而双层细胞种植组仅有 2 7%EC脱落 ,双层细胞种植组细胞保存率明显高于单纯EC种植组 (P <0 0 1)。低流速预灌注未能改善EC的保存率。
Objective To improve cell retention on the graft with dual cell seeding technique, adding a layer of smooth muscle cells (SMCs) between the graft and endothelial cells (ECs). Methods Polytetrafluoroethylene (PTFE) grafts pretreated with fibronectin were seeded with SMC followed by ECs one day later and exposure to an in vitro flow system. The number of ECs on grafts was counted under microscope. Results After exposure to the flow for 1 hour, 61% of the ECs lost when the ECs were seeded alone, whereas only 27% of the ECs lost when the ECs were seeded on the top of SMCs. Preconditioning of a cell seeded graft in a low rate flow did not improve cell retention when late exposure to a higher rate flow. Conclusion This in vitro study indicates that using SMCs as a media layer between ECs and graft surface can improve the retention of seeded ECs on PTFE graft.
出处
《中华外科杂志》
CAS
CSCD
北大核心
2003年第2期143-145,共3页
Chinese Journal of Surgery
基金
浙江省科技厅科研基金资助项目(991110 0 5 2 )
浙江省医药卫生科学研究基金资助项目(2 0 0 0A110 )
关键词
人工血管
血管内皮
细胞种植
平滑肌细胞
Blood vessel prosthesis
Endothelium, vascular
Muscle, smooth, vascular
Cell seeding
