摘要
运动性疲劳与细胞凋亡时机体内环境的改变有雷同之处 ,通过对疲劳与细胞凋亡关系的研究 ,为运动性疲劳的发生机制提供理论依据。采用流式细胞仪 (FCM)检测肝、肌细胞Ca2 + 浓度及DNA倍体分析 ,末端转移酶标记 (TUNEL)技术检测细胞凋亡。结果表明 ,运动性疲劳后肌、肝细胞中Ca2 + 浓度显著增高P <0 .0 1;FCM检测肌、肝细胞实验组均出现凋亡峰 ;TUNEL染色显示 ,疲劳时肌、肝组织呈棕黄色的凋亡细胞比例明显增多 ;各组织SOD活性 12d组显著下降P <0 .0 1,MDA数量 12d组非常显著升高P <0 .0 1;研究认为 :细胞内Ca2 + 浓度显著增多、SOD与MDA比值变化是决定细胞凋亡或坏死的主要原因 ,细胞凋亡与运动性疲劳同步发生 ,诱导细胞发生凋亡的因素均会导致疲劳。
Exercise induced fatigue is similar to the changes of internal environment of the body during apoptosis. The relationship between fatigue and apoptosis was studied to provide theoretical basis for explaining the mechanism of exercise induced fatigue. The flow cytometry (FCM) was used to examine the Ca 2+ concentration in myocytes and hepatocytes and the DNA double analysis was conducted. The apoptosis was examined by using the TUNEL technique. It was indicated that the Ca 2+ concentration in myocytes and hepatocytes remarkably increased after exercise induced fatigue (P <0.01). The peak apoptosis was found in the experimental group when the myocytes and hepatocytes were examined with the FCM. The TUNEL stained preparation showed that the ratio of the brown apoptosis cells in muscular and hepatic tissues increased remarkably during fatigue. The superoxide dismutase (SOD) activities of various tissues decreased obviously in the group, training for 12 days ( P <0.01), but the amount of malonaldehyde (MDA) in that group increased remarkably (P < 0.01 ). It was considered that the obvious increase of Ca 2+ concentration in cells and the change of the SOD/MDA ratio are the main reasons for determining apoptosis. The apoptosis synchronically occurring with exercise induced fatigue and factors inducing apoptosis are able to lead to fatigue.
出处
《北京体育大学学报》
CSSCI
北大核心
2002年第6期766-768,共3页
Journal of Beijing Sport University
基金
国家体育总局课题 :体科字 [1 997] 1 78号
六科大主题报告
