摘要
目的 探讨肿瘤病人外周血单个核细胞 (PBMC)中T细胞核仁嗜银蛋白 (AgNORs)变化和辅助性T(Th)细胞亚群变化的关系及其临床意义。方法 PBMC经多克隆T细胞活化剂刺激 ,以图像分析法检测AgNORs区面积与细胞核仁面积的百分比 (I.S % ) ;PBMC被离子霉素和佛波醇酯活化 5h后 ,以酶联免疫斑点法 (ELISPOT)检测γ干扰素 (IFNγ)和白细胞介素 4(IL 4)产生细胞的频度( % ) ;以离子霉素加佛波醇酯刺激 2 4h ,用ELISA试剂盒检测培养上清液中的IFNγ 和IL 4水平。结果86例肿瘤病人和 44名正常人 ,PBMC中活化T细胞AgNORs表达分别为 ( 4 90± 1 2 0 )I S %和 ( 7 82±1 2 8)I S % ,肿瘤组下降非常显著 (P <0 0 5 ) ;肿瘤组PBMC中Th2细胞频度显著上升 ,Th/Th2比值显著下降 ;同时 ,肿瘤组PBMC产生IFNγ 和IL 4水平发生相应变化 ,变化方式与Th亚群变化相似 ;肺癌转移组与未转移组、复发组与未复发组相比 ,Th1细胞数、Th1/Th2之比和IFNγ 的产生下降非常显著 ;在肿瘤组AgNORs表达与Th1细胞频度、Th1/Th2比值、IFNγ 产生和肿瘤病人生活质量评分呈正相关。结论 肿瘤病人PBMC中T细胞表达AgNORs下降 ,与患者Th1细胞功能下降呈正相关。
Objective To investigate the changes of silver-stainng protein of nuclear organizer regions(AgNORs)expression of T lymphocytes and helper T (Th) cell subsets in peripheral mononuclear cells (PBMC) from cancer patients and to analyze the relationship between them.Methods AgNORs expression in T cells activated by a multiple clonal activator was examined using a special image system. After activation of PBMC with ionomycin plus PMA for 5 h, both interferon-γ (IFN γ) and interleukin-4 (IL-4)producing cell frequencies were detected by enzyme-linked immunospot assay (ELISPOT). The stimulated with ionomycin plus PMA for 24 h, the IFN γ-and IL-4-production were assayed by ELISA.Resuts AgNORs expression of activated T cells in PBMC from cancer patients (n=86) and healthy persons (n=44) was (4.90±1.20) I.S% and (7.82±1.28) I.S% respectively. Th2 frequency was significantly higher and the ratio of Th1 to Th2 frequency was more lower in cancer patient group. Moreover, the IFN γ and IL-4 production in cancer patient group were changed in the way similar to Th subset frequencies. In lung cancer patients the AgNORs expression, Th1 frequency, Th1/Th2 ratio and IFN γ production were significantly decreased in metastasis or in recurrence status,AgNORs expression in cancer patients correlated with their Th1 frequencies, Th1/Th2 ratio, IFN γ production levels and Karnofsky′s estimators.Conclusions In analyzed cancer patient cases, the expression of AgNORs and the function of Th1 cell were significantly decreased and correlated.
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2003年第1期34-36,共3页
Chinese Journal of Laboratory Medicine
