摘要
目的 探讨转化生长因子_β1 (TGF_β1 )在静张应力环境下对髁突软骨细胞增殖效应的影响。方法 (1)将传代培养至第 4代大鼠下颌髁突软骨细胞在 12孔培养板上培养 ,采用流式细胞仪测定加入不同浓度TGF_β1 (0 1、1、10ng ml) 0、6、12、18、2 4h后 ,对髁突软骨细胞增殖活性 (PI值 )的影响。 (2 )将第 4代大鼠下颌髁突软骨细胞在细胞膜式张应力施加装置上培养 ,检测不同浓度TGF_β1 及 5kPa静张应力联合应用 0、6、12h后 ,对髁突软骨细胞增殖活性(PI值 )的影响。结果 不同浓度TGF_β1 (0 1、1、10ng ml)在模拟生理环境下以剂量依赖型的方式整体促进了髁突软骨细胞增殖效应 ,对髁突软骨细胞的增殖活性调节作用主要在 12～ 18h段 ,增殖峰值在 18h左右 ;在 5kPa静张应力联合TGF_β1 刺激下培养较TGF_β1 的单独效应具有更强的促髁突软骨细胞增殖作用。结论 静张应力联合TGF_β1
Objective\ The purpose of this study was to investigate effects of static tension_stress and TGF_β\-1 on proliferation of mandibular condylar chondrocytes in vitro.Methods\ The fourth_passage chondrocytes were harvested from the mandibular condyles of 2_week_old SD rats for this study, and a cellular static tension_stress device was used to apply stress on cells. The effects of continuous static tension_stress and/or transforming growth factor_β\-1 (TGF_β\-1) on the proliferation of mandibular condylar chondrocytes were examined using flow cytometry. The experiment was divided into two parts. The first part consisted of 100 specimens which were divided into 20 groups with different TGF_β\-1 dosage (0 ng/ml, 0.1 ng/ml, 1 ng/ml and 10 ng/ml) for 0, 6, 12, 18 and 24 hours respectively. The second part consisted of 30 specimens which were divided into six groups under continuous static tension_stress (5 kPa) and different TGF_β\-1 doseage (0.1 ng/ml, 1 ng/ml, 10 ng/ml) for 0, 6 and 12 hours. Multivariable analyses were conducted to test for associations between proliferation of mandibular condylar chondrocytes and TGF_β\-1 and/or different stresses.Results\ The results showed that TGF_β\-1 had a mitogenic effect on rat mandibular condyle at the concentrations of 0.1, 1 and 10 ng/ml, and the mitogenic effects of TGF_β\-1 on condylar chondrocytes were demonstrated in 12 to 18 hours after application of stresses, and the peak of mitogenic effects appeared at 18 hour (P<0.05). The most active mitogenesis happened in the group with continuous static tension_stress (5 kPa) combined with TGF_β\-1.Conclusion\ These results prove mechanical stimulates and TGF_β\-1 in vitro could influence and regulate the growth of condylar chondrocytes.\;
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2003年第1期61-63,73,共4页
West China Journal of Stomatology
基金
国家自然科学基金资助项目 (编号 39870 2 313990 0 1 66)
关键词
静张应力
转化生长因子-β1
髁突软骨细胞
增殖活性
功能矫治
正畸治疗
static tension_stress
\ transforming growth factor_β\-1
\ mandibular condylar chondrocytes
\ proliferation
\ functional orthopedics
