摘要
目的 建立人骨骼肌干细胞体外分裂分化的模式。方法 人工流产胎儿肌肉组织用胰酶和胶原酶联合消化后 ,经percoll密度梯度离心分离骨骼肌干细胞 (又称卫星细胞 )。并以Matrigel为基质 ,在体外培养条件下观察其分裂与分化。结果 经percoll密度梯度离心 ,能将干细胞与成纤维细胞分离 ,纯度达 80 %以上。干细胞具有分裂、分化的潜能 ,在体外由成肌细胞增殖 ,经彼此融合成为肌管细胞 ,最终形成多核且具有收缩能力的骨骼肌细胞。上述分裂、分化过程历时 2 1天 ,较啮齿类长。结论 人胎儿骨骼肌干细胞在体外具有分裂、分化潜能。因此 。
Objective To develope cell culture system for the study of muscular stem cell (satellite cell)proliferation and differentiation Methods The stem cells of human muscle cells were prepared by percoll gradient centrifugation and Matrigel was used as cultivation substrate Results It was found that during 20 days of cultivation the satellite cell proliferation and differentiation was from myoblasts ,myotubes to muscle cells during 20 days Conclusion This novel procedure ,along with the simple culture technique ,could be used in cell or gene therapy
出处
《临床和实验医学杂志》
2002年第4期222-224,共3页
Journal of Clinical and Experimental Medicine
关键词
骨骼肌干细胞
实验研究
细胞培养
Human muscular stem cell ( Satellite cell)
Cell culture
