摘要
目的 :观察华蟾素对细胞生长及凋亡的影响。方法 :应用体外细胞培养技术、MTT测定、集落形成试验、电镜观察及DNA琼脂糖凝胶电泳等方法。结果 :华蟾素对MGC 80 3细胞的生长有显著抑制作用 ,能抑制细胞的集落形成 ,MTT法测定 ,其IC50 约为 0 16 μg/ml;电镜下观察到细胞呈凋亡特征 ;琼脂糖凝胶电泳显示DNA呈梯状降解。 结论 :华蟾素能抑制MGC- 80 - 3细胞生长 ,药物作用方式在较高浓度时以细胞毒作用为主 ,在较低浓度时以诱导细胞凋亡为主。诱导细胞凋亡是华蟾素抗肿瘤的重要方式之一。
Objective:The effect of cinobufacini on the growth of human gastric neoplasm cell line MGC 80 3 is studied to find o ut the mechanism of its anticancer effect.Methods:Techniques of cell culture in vitro,MTT assay,c olony formation assay,electronmicroscopy detection and DNA gelelectrophoresis w ere used.Results:Cinobufacini can apparently inhibit the growth an d colony formation of MGC-80-3 cell line and its IC 50 is about 0 16 μg /ml detected by MTT assay.The cells showed characters of apoptosis observed by electronmicroscopy.Gelelectrophoresis of DNA showed change of 'ladder'pa ttern.Conclusion:Cinobufacini can inhibit the growth of MGC 80 3 cell line. The mode of cell death is cellulotoxic under high dose of the dr ug and apoptotic under the lower dose.Inducing cell apoptosis is one of the im portant mechanisms of cinobufacini anticancer.
出处
《临床肿瘤学杂志》
CAS
2003年第1期33-35,37,共4页
Chinese Clinical Oncology
