摘要
目的 :探讨肺癌胸水中肿瘤浸润淋巴细胞 (TIL)的生物学活性 ,便于更好地应用于临床。方法 :用白细胞介素2 (IL 2 )诱导培养肺癌胸水TIL ,按常规法计数TIL细胞增殖量 ,采用流式细胞仪分析TIL细胞表型及MTT法检测其杀瘤活性。结果 :经IL 2诱导培养TIL 2 1天后 :TIL增殖大于 10 0 0倍的患者有 17例占 77%。CD3差异无显著性 (P >0 0 5 ) ,CD4、CD8差异有显著性 (P <0 0 1) ,而CD4 /CD8比值高达 3 5 3± 0 82 ,并且在 2 1天时TIL具有较高的细胞毒性。结论 :肺癌胸水中的TIL体外诱导培养 2 1天时TIL具有较强的生物活性 。
Objective:To study the specific prope rt y of the biological activity about tumour infiltrating lymphocytes (TIL) on mali gnant pleural effusion of lung cancer, to apply on clinic for better.Methods:To use the interleukin 2(IL 2) to culture and gu ide the tumour infiltrating lymphocytes on malignant pleural effusion, to count TILs proliferative index according to the routine way, analyze TILs epitope to use the flow cytometry (FACS) and test thd cytotoxic activity by the MTT method s.Results:After 21 days, accroding to apply IL 2 (100U/m l) to culture and guide TILs, the patients of the TILs increment number over 1 000 times was 77% .CD3 was no difference outstanding ( P >0 05), but CD4, CD8 were significantly higher than other days ( P <0 01), then CD4/CD8 was a high level of 3 53±0.82, and TILs were provided with higher cytotoxic act ivity.Conclusion:It will be acquired the reliable curative effe ction on malignant pleural effusion, this TILs had gained higher biological act ivity when the TILs on malignant pleural effusion were cultured in the 21st day .
出处
《临床肿瘤学杂志》
CAS
2003年第1期9-10,13,共3页
Chinese Clinical Oncology
关键词
肺癌
胸腔积液
肿瘤浸润淋巴细胞
增殖力
表型分析
杀伤细胞活性
Lung cancer
Pleural effusion
Tum our infiltrating lymphocytes
Proliferative index
Epitope analysis
Cyto toxic activity
