摘要
目的 研究丙丁酚对兔经皮腔内血管成形术 (PTA)后内膜增生及管壁细胞周期蛋白表达的影响。方法 新西兰白兔随机分为假手术对照组 ,PTA术后 2周组及 PTA术加丙丁酚防治 3周组和 5周组。治疗组在 PTA术前1周给药 ,术后继续给药 2周和 4周 ,分别为丙丁酚防治 3周组和 5周组。取各组实验动脉段石蜡切片 ,采用计算机图象分析系统分别测出中膜面积 (MA)、新生内膜面积 (NEA) ,并计算新生内膜面积与中膜面积的比率 (NEA /MA)。用免疫组化测周期素 D1 (cyclin D1 )、周期素依赖激酶 4(cyclin- dependent kinase 4,cdk4)及增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)的表达水平。结果 PTA术后 2周组 ,内膜增生显著 ,cdk4、cyclin D1 和PCNA均在内膜层表达 ,而中膜表达较少或几乎检测不到。与 PTA术后 2周组相比 ,丙丁酚防治 3周组新生内膜减少达 71.3 % (P<0 .0 1) ,cdk4、cyclin D1 和 PCNA阳性表达指数分别低 69.9%、73 .7%和 79.6% (P均 <0 .0 1)。结论 丙丁酚防治 3周对兔血管损伤后内膜增生抑制作用显著 ,同时伴有 cdk4、cyclin D1 和 PCNA阳性表达下降。
Objective To investigate the effect of probucol on neointimal hyperplasia and expression of related cell cycle proteins after percutaneous transluminal angioplasty (PTA) in rabbits. Methods Rabbits were randomly assigned to a sham operation group, PTA 2 weeks group,PTA plus probucol 3 weeks group and probucol 5 weeks group. Probucol was given orally 1g/d from 1 week prior to PTA to the time of killing(2 or 4 weeks after PTA, that's probucol 3 weeks group and probucol 5 weeks group respectively).Then the medial area (MA), neointimal area(NEA) and neointimal area(NEA)/medial area(MA) were analyzed by computerized digitizer system. The expression of cyclin dependent kinase4(cdk4) , cyclin D 1 and proliferating cell nuclear antigen(PCNA) were measured by immunohistochemical technique and computer image analyzer. Results In PTA 2 weeks group ,cdk4, cyclin D 1 and PCNA expressed mainly in the neointima but with low or undetected expression in the media,the expression of cdk4,cyclin D 1 and PCNA increased with the intimal thickening.Compared with PTA 2 weeks group, the neointimal area reduced by 71.3%( P <0.01),and the positive expression indexes of cdk4, cyclin D 1 and PCNA decreased by 69.9%, 73.7% and 79.6% respectively (all P <0.01) in PTA plus probucol 3 weeks group. Conclusion Probucol can effectively inhibit neointimal thickening after angioplasty,which is probably due to decrease in the expression of cdk4, cyclin D 1 and PCNA.
出处
《中国心血管杂志》
2003年第1期1-3,14,共4页
Chinese Journal of Cardiovascular Medicine
基金
国家自然科学基金 (编号 :39970 847)
湖南省自然科学基金 (编号 :99JY2 0 0 32 )项目
