摘要
血管生成素 (angiopoietin)是近年来发现的与血管生成、抑制密切相关的新分子。为研究其在肿瘤血管生成及转移中的作用 ,利用PCR技术从人胎盘组织中得到血管生成素 1基因 ,并亚克隆至pRSETC原核表达载体及pcDNA3 1 V5 HisC真核表达载体 ,成功地构建了原核及正反义真核表达载体 ,为下一步研究其对消化道肿瘤血管生成的影响打下了基础。
Angiopoietin is a novel angiogenesis related molecule. To investigate its roles in tumor angiogenesis and metastasis,angiopoietin 1 gene was obtained from human placental tissue by reverse transcription polymerase chain reaction (RT PCR). The sense, antisense eukaryotic expression vector pcDNA3.1/hangiopoietin1(+), antisense eakaryotic expression vector pcDNA3.1/hangiopoietin1(-), and prokaryotic expression vector pRSETC hangiopoietin1 were successfully constructed by directional cloning the target gene into pcDNA3.1 V5 His C and pRSET C vectors, respectively. This lays good foundation for further study on the roles of angiopoietin 1 in angiogenesis in tumors of the digestive tract.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2003年第2期158-160,共3页
Medical Journal of Chinese People's Liberation Army
基金
国家自然科学基金 (编号 30 1 30 2 60 )
军队"十五"重点基金 (编号0 1 2 0 87)资助课题
