摘要
目的 :观察不同程度的慢性肾功能不全 (CRI)患者体内脂质过氧化物和抗氧化酶活性的改变 ,探讨氧化损伤状态和抗氧化系统变化在 CRI发病机制中的作用 ,以及它们与肾功能改变的关系。方法 :采用化学比色法 ,对 36例 CRI患者、12例血液透析患者和 16例正常人的血清丙二醛 (MDA)、谷胱甘肽过氧化物酶(GSH Px)及血清超氧化物歧化酶 (SOD)的含量进行了检测。结果 :36例 CRI患者、12例血液透析患者的血清 MDA水平均明显高于正常对照组〔(4.0 6± 0 .6 7) μmol/ L〕,有显著性差异 (P<0 .0 1) ;GSH Px均明显低于正常对照组〔(12 0 .6 3± 2 7.5 7)× 10 4 U/ L〕,有显著性差异 (P<0 .0 1) ,血清 SOD亦明显高于正常对照组〔(110 .30± 18.6 0 ) k NU / L〕,有显著性差异 (P<0 .0 1)。 36例 CRI患者 SOD、GSH Px和 MDA含量与内生肌酐清除率 (CCr)均呈密切相关 (r G=0 .6 8,P<0 .0 1;r M=0 .5 2 ,P<0 .0 1;r S=0 .4 4 ,P<0 .0 5 )。结论 :自由基、脂质过氧化损伤和抗氧化系统的缺陷在慢性肾功能不全的发病机制中起重要作用 ,并且与肾功能的改变密切相关 ,这些酶活性还可作为临床判断病情严重程度的指标。
Objective:To observe the changes of lipid peroxidation and antioxidative enzyme activities of chronic renal insufficiency (CRI) and to explore their effects on the pathogenesis of CRI.Methods:Serum levels of malondialdehyde (MDA),serum glutathione peroxidase (GSHPx) and serum superoxide dismutase (SOD) in 36 nondialyzed patients with various degrees and 12 hemodialyzed(HD) were determined with the method of chemistry colorimetry.Results:Thirtysix CRI and 12 HD patients included in the study had higher SOD activities when compared with the controls〔(110 30±18 60) kNU/L〕.They also had lower serum GSHPx activity than the controls〔(120 63±27 57)×10 4 U/L〕.Serum MDA levels in the patients were higher than that in the controls〔(4 06±0 67) μmol/L〕.Serum SOD,GSHPx and MDA concentrations in 36 nondialyzed patients with various degrees were also correlated with creatinine clearance( r G=0 68, P <0 01; r M=0 52, P <0 01; r S=0 44, P <0 05).Conclusion:Free radicals,lipid peroxidation and the abnormality in antioxidative system may play an important role in the pathogenesis of CRI and correlated with impaired renal function,and that they can be regarded as important clinical indexes to inflect the severity of chronic renal insufficiency.
出处
《中国危重病急救医学》
CAS
CSCD
2003年第2期88-90,共3页
Chinese Critical Care Medicine
基金
山东省医药卫生科研项目 (2 0 0 1CA2 EFB)
关键词
氧化损伤
氧自由基
肾功能不全
慢性
oxidative stress
oxygen free radicals
chronic renal insufficiency
