摘要
根据已报告的传染性法氏囊病病毒(Infectious bursal disease virus,IBDV)cDNA序列,设计引物,用RT-PCR扩增CH(鸡)、DU(鸭)、GE(鹅)和SP(麻雀)四种不同源IBDV分离株的vp2基因高变区。核酸序列测定分析表明,四种不同源IBDV分离株vp2基因高变区的同源性为97％.推导编码蛋白氨基酸序列的同源性98％,两个亲水区和七肽区的氨基酸序列完全一致。本研究结果提示,自然感染IBDV的鸭、鹅和麻雀不仅可成为病毒携带者或传染源,而且在病毒变异中起一定作用。
According to the published cDNA sequences of Infectious bursal disease virus(IBDV), a pair of primers to variable region in VP2 were designed for cDNA synthesis and PCR amplification of four IBDV isolated from different bird species, CH, DU, GE and SP. The nucleotide sequences determined have been compared to each other. The four isolates are closely related, the homology of the four isolates being 97% at the nucleotied level and 98% at the amino acid level.Their two hydrophilic regions and one heptapeptide were identical at the amino acid level. The results revealed that the ducks,geese and sparrows naturally infected with IBDV not only could become the carriers or reservoirs in transmission but may take certain effect on the variation of IBDV.
出处
《中国病毒学》
CSCD
2003年第1期68-71,共4页
Virologica Sinica
基金
江苏省自然科学基金资助项目(BK97187)
关键词
麻雀
鸭
鹅
传染性法氏囊病病毒
VP2
序列分析
Sparrow
Duck
Goose
Infectious bursal disease virus
VP2
Sequence analysis
