摘要
应用光镜、电镜和组织化学技术，对 １０ 只成年海狸鼠消化管的食管、胃底、小肠和大肠壁的显微和亚显微结构进行了研究。结果显示，在 Ｈ Ｅ 染色，海狸鼠食管粘膜上皮细胞角化程度较高，胃底腺壁细胞的数量几乎与主细胞的相等或稍多，小肠肠腺底部含有大量潘氏细胞； Ｓｃｈｉｆｆ 氏染色，胃粘膜的上皮细胞、胃底腺的颈粘液细胞、十二指肠腺的粘液细胞、肠腺的潘氏细胞和杯状细胞均为阳性； Ｍ ａｓｓｏｎｆｏｎｔａｎａ 氨银法染色，在胃底腺和肠腺内均见有银亲合细胞。扫描电镜下，胃粘膜表面见有较多胃小凹，小肠上皮细胞的微绒毛形成许多指状突起。透射电镜观察，胃底部壁细胞中线粒体、滑面内质网、细胞内小管发达，小肠上皮细胞的微绒毛密集排列，细胞间见有连接复合体结构，胃底腺和肠腺内见有散在分布的内分泌细胞。
The optical microscope, electron microscope and histochemistry were used as basic techniques to study microscopic structure and submicroscopic structure of digestive tubes in 10 adult myocastor coypus. The results were as follows: 1. Many esophageal peithelial cells showed keratinized. The numbers of parietal cells were equal to or slightly more than those of chief cells in fundus glands. There were many Paneth′s cells in the intestinal gland bottoms of small intestine. The positive reactions of Periodic Acid Schiff (PAS) appeared in epithelial cells of stomach tunica mucosa, mucous neck cells of fundus glands, mucous cells of glandulae duodenales, Paneth′s cells and goblet cells of intestinal glands in small intestine. There were argyrophil endocrine cells in fundus glands and intestinal glands. Under scanning electron microscope(SEM), there were many gastric pits in the surface of stomach tunica mucosa, and many microvilli in the surface of small intestine. Under transmission electron microscope(TEM), there were lots of mitochondra, smooth endoplasmic reticulum (SER) and intracellular canaliculi in the parietal cells of fundus glands. The microvilli of tunica mucosa of small intestine were arranged densely. There were junctional complexes between the epithelial cells of small intestinal tunica mucosa. There were the scattered endocrine cells in fundus glands and intestinal glands.
出处
《中国兽医学报》
CAS
CSCD
北大核心
1999年第5期508-510,共3页
Chinese Journal of Veterinary Science
关键词
消化管
显微结构
亚显微结构
海狸鼠
digestive tube
microscopic structure
submicroscopic structure
myocastor coypus
