摘要
根据已发表的基因序列设计了 1对PCR引物 ,以伪狂犬病病毒Ea株 (pseudorabiesvirusEa ,PRVEa)基因组DNA为模板 ,扩增出一大小为 5 11bp的片段。通过酶切和测序证实 ,该基因为伪狂犬病病毒Ea株的又一糖蛋白基因 ,即gL基因。Blast软件分析表明 ,该基因与Kaplan株核苷酸序列的同源性为 94% ,氨基酸序列的同源性为 95 %。将测序结果输入GenBank ,获得登录号AF44 845 6。
A 511 bp gene segment was amplified by PCR with the template of pseudorabies virus Ea strain genome DNA and a pair of primers designed acording to the published gene sequence. Restriction nuclease enzyme digestion and sequence analysis indicated that the gene segment is gL gene . There are several mutations, which results in the 94% DNA identifity to PRV strain Kaplan. Further analysis demonstrated in PRV Ea gL gene there is 95% amino acid identifity. The gL gene sequence was submitted to GenBank, and the accession number is AF448456.
出处
《中国兽医科技》
CSCD
北大核心
2002年第11期5-7,共3页
Chinese Journal of Veterinary Science and Technology
基金
国家自然科学基金资助项目 (39970 559)
