摘要
以6 0 Coγ射线照射体外培养的人乳腺癌MCF - 7细胞 ,运用流式细胞术探讨了受照射后细胞周期进程的变化规律。结果表明 ,MCF - 7细胞受 5 .0Gyγ照射后呈现短暂的S期阻滞(持续约 6h) ,主要为G2 期阻滞 (持续约 6 3h)。S期和G2 期阻滞高峰分别出现在照射后 9h和18h ,阻滞峰值分别达正常值的 1.6倍和 6 .2倍。照射后 9h和照射后 18h的剂量—效应曲线截然不同。照射后 9hS期阻滞和照射后 18hG2 期阻滞均与照射剂量呈现明显剂量—效应关系。
To investigate the effects of ionizing radiation on cell cycle progress of tumor cell lines, the human breast cancer MCF-7 cell line cultured in vitro was exposed to 60 Co γ rays and the alterations in cell cycle progress after irradiation were measured by flow cytometry. The results indicated that the MCF-7 cells showed a transient S arrest continuing for about 6h and an obvious G 2 arrest continuing for about 63h after irradiation with 5.0Gy γ rays. S and G 2 arrest culminated at 9h and 18h respectively after irradiation and the peak values of S and G 2 arrest reached respectively 1.6 times and 6.2 times as many as normal value. The dose-effect curve examined 9h after irradiation was quite different from that examined 18h after irradiation. Both of the S arrest at 9h after irradiation and the G 2 arrest at 18h after irradiation presented significant relationship with irradiation dose.
出处
《辐射研究与辐射工艺学报》
CAS
CSCD
北大核心
2002年第4期281-283,共3页
Journal of Radiation Research and Radiation Processing
基金
北京大学基础医学院凯华基金资助
