摘要
本研究采用一种新的细胞DNA双链断裂检测方法——脉冲电场电泳法,检测了两株来源相同而辐射敏感性不同的细胞SR-1和SX-9的X线照射所致DNA双链断裂的产生和修复。结果表明两株细胞的DNA双链断裂产生没有差别,而辐射敏感细胞SX-9的DNA双链断裂重接修复能力明显低于SR-1细胞,本文对此结果与细胞辐射敏感性的关系进行了讨论。
The intinial amount and repair of DNA double strand breaks (DSBs)
in the radiosensitive cell SX-9 and wild-type cell SR-1 irradiated by X ray were
detected by pulsed field gel electrophoresis. The results showed that the extent of DNA
DSBs was similar in these two cell lines under same X ray doses, but the rejoining
repairca pability of DNA DSBs in SX-9 cells was significantly lower than that in SR-1
cells. Results suggested that the increased radiosensitivity in SX-9 cells could be corr-
elated with a defect in the repair of DNA DSBs.
出处
《辐射研究与辐射工艺学报》
CAS
CSCD
北大核心
1992年第1期44-47,共4页
Journal of Radiation Research and Radiation Processing
关键词
DNA双链断裂
辐射敏感性
癌细胞
DNA double strand breaks (DSBs)
Radiosensitivity
Pulsed field gel electrophoresis
Radiosensitive mutant
